Faculty

Madhu Khanna 


 

CURRICULUM VITAE

Address

Work

Professor
Department of Virology

VP Chest Institute

Delhi University, Delhi 110007

New Delhi -110012 

India


Telephone:      +91-11-27402432 (W)

 

Home

C 34, Pusa Campus

New Delhi -110012

India

Telephone:      +91-124- 4074922

Mobile: +91-9958524922

Email: madhukhanna@hotmail.com

 

 

Personal Details

Marital status: Married
Nationality: Indian

 

 

Education

Ph.D. (Lucknow University) from KG. Medical College, Department of Virology Thesis supervisor Professor UC Chaturvedi, Head, Department of Microbiology, KG Medical College Lucknow, India.  

 

Thesis title Purification and characterization of dengue virus induced cytotoxic factor.

 

Research Interests  

·       Molecular diagnosis of viruses

·       Pathogenesis of virus

·       Antiviral strategies

 

Professional Affiliations

·       Life member: Indian Immunology Society since 1995

·       Life member: Biotechnology Society of India 2004

·       Life member: Influenza foundation of India 2010

·       International member: ICEID

·       International member: ISIRV 2011

·       International member: ESCMID 2016

·       Life member: Indian Virological Society 2013

·       CPCSEA-IAEC Link Nominee for Indira Gandhi National Open University, Maiden Garhi, New Delhi from25th July 2014 to 24th July 2017

·       CPCSEA-IAEC member for Bio-Med Pvt. Ltd., Bulandshahar, Ghaziabad

·       CPCSEA-IAEC Member Link Nominee for AmityUniversity Campus, Noida

·       CPCSEA-IAEC Member Link Nominee for Jamia Hamdard University, New Delhi

·       CPCSEA-IAEC Member for Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi from 18.02.2014 to 11.08.2017

 

2. Teaching Experience

 

Name of the Institution

Designation with pay scale

Nature of post

Classes taught/type of duties

Period

From To

Total Experience

Vallabhbhai Patel Chest Institute

 

Professor

Permanent

Research / Teaching

MD and PhD

 

Microbiology

31.12.2008

 

till date

Total experience

18years 3 months

6 years 10 months (Present post)

Vallabhbhai Patel Chest Institute

Associate Professor

 

Permanent

Research / Teaching

MD Microbiology & M.Sc. Virology

(At ACBR)

20.10.2004          to 

  30.12.2008

 

4 years 2 months

AIIMS Department of BiochemistryNew Delhi.

Research Officer

Temporary

Research & Teaching

Undergraduates (B.Sc)

1st May 1996 to                     23 June 1997

1 year

& 1 month

AIIMS Department of Biochemistry

Research Associate

Temporary

Research &

Teaching

Undergraduates (B.Sc.)

1st Sept 1992 to

30 April 1996

3 years  & 7 months

 

 

Conference Organized 

PhD/MD students guided

Ø  8  PhD & 4 MD (Dissertation) theses awarded

Ø  1 pursuing PhD

Ø  13 MSc Dissertation Student

 

 

Award and Honours  

 

1.    Editor of following journals:

§    International Journal of Immunology Research

§    Journal of Virology Research

§    Asthma & Bronchitis journal since 2015

2.   Secretary General, Biotechnology Society of India

3.   Joint Secretary, International Association of Medical and Pharmaceutical  Virologists

4.  Bronze award (AVG Award) for the best paper in Ditan International Conference on Infectious Diseases, Beijing, China on July, 30-Aug 2, 2009.                                                     

5.   Young Scientist award, European Scientific Working Committee (ESWI), 14-17 Sept, 2008, Portugal.

6.   Young investigator award in “Options for the Control of Influenza VI” Ontario, Canada 17-23 June, 2007(Oral presentation).

 

7.  BOYSCAST Fellowship from Department of Science and Technology, GOI in the 2000, to work at WHO Center for reference and research center Australia, Melbourne.

 

8. Shakuntala Amir Chand Award of Indian Council of Medical Research (ICMR) for the year 1992 for outstanding research work in the field of "Cytokine Mediated Pathology of Dengue-virus Infection".

 

9.  DST Young Scientist independent scheme on Characterization of drug resistant clinical isolates of M. tuberculosis by PCR analysis. Cost Rs. 5,00 000

 

10.   CSIR Individual fellowship of Research Associate for the project entitled: Spontaneous Release of Interleukin-2 and Generation of Superoxide In Active Pulmonary Tuberculosis

 

11.    BEST POSTER AWARD during the XXII Annual Conference of Indian Immunology Society & National Symposium on Immunology of Infectious Disease, held during 15-17 Dec. 95, JNU, New Delhi, India.

 

12.    Bill & Melinda Gates Foundation’s ‘Global Health Award’ to attend Keystone symposia on “Advancing vaccine in the genomic era” held from Oct. 31- Nov. 4, 2013 at Brazil.

 

Reviewer of following Journals

·         IJMR

·         IJCD

·         Virology Journal

·         Infection Genetics &Evolution

·         Epidemiology  & Infection

·         European Respiratory Journal

·         Plos One

·         Journal of General Virology

·         Infection Genetics & Evolution

·         Epidemiology & Infection

·         Journal of Virology

·         African Journal of Microbiology Research

 

 

International Project

 

1.      Profile of antibody responses and duration of protection following influenza vaccination for adults >65 years old

 

APACI

2013

USD 30,604

Ongoing

 

National Projects (Sponsored by GOI)

 

 

Name of the Project

Funding  Agency

 

Date of Sanction

 

Total Approved

Cost of the Project (in Rs.)

Status

 

2.    Evaluation of antiviral activity of medicinal plants against influenza A virus

AYUSH

Jan 2014

25 Lakhs

Ongoing

 

3.    To study the heterosubtypic immunity provided by pandemic influenza A H1N1 (2009) virus infected cells.

CSIR

Oct 2013

30 Lakhs

Ongoing

 

4.    Recombinant monoclonal antibodies against influenza A virus and pandemic     influenza 2009 (PI)

 

DST

 

 

Jan. 2011

 

43.5 Lakhs

 

Completed

 

5.    Study of antigenic diversity and cross reactive antibody generation to influenza virus in human samples (PI)

 

DRDO

 

Apr. 2011

 

45.2 Lakhs

 

Completed

 

 

6.      A combinatorial antiviral approach against influenza A virus using ribozymes and siRNAs ( PI)

 

DBT

 

Nov. 2006

 

   

40.2 Lakhs

 

 

Completed

 

 

7.      A Study of Viral Replication Inhibition by Down Regulation of NS1 Gene of    Influenza A Virus (PI)

 

 

 

CSIR

 

Oct 2007-Oct 2010

 

13 Lakhs

 

Completed

 

 

8.      Multi-Site Monitoring of Human Influenza Viruses in India Phase-I”(PI)

 

ICMR

 

Sep. 2006-28 Feb. 2010

 

1 Crore

 

Completed

9.      Typing and subtyping of influenza virus by RT-PCR (PI)

 

DST

 

Dec.1998

 

37.5 Lakhs

 

Completed

10. Study of Virological and biochemical regulatory mechanism of influenza virus induced apoptosis in murine model of allergic asthma (PI)

 

CSIR

 

 

Feb 2003

 

 

 

 

13 Lakhs

 

 

 

 

 

Completed

11.  Genetic analysis of influenza virus by molecular techniques (PI)

 

DST

 

Nov 2003

 

18 Lakhs

 

Completed

           

 

 

 

 

International Research Trainings

 

1.      3rd ICGEB workshop on Human RNA Viruses, held at Argentina, 3-5th Apr. 2012.

 

2.      The Baltic Summer School, International Training Course on "Current Trends In Immunology And Signal Transduction" Including Practical Course On Immunology And Molecular Biology Techniques At University of Kiel, Germany from Sept.17-Oct.8, 2001.

 

3.      International Training Course on Cellular and Molecular Biology sponsored by International Research Organization at Wiezmann Institute, Israel on 10 Dec.1994 to 23 Dec.1994.  

 

 

National Trainings:

 

 

  1. IUIS Advance Course on Basic Immunology held at All India Institute Medical Sciences New Delhi on Nov. 22-27, 1993.
  2. Committee for the Purpose of Control and Supervision of Experiments on Animal     (CPCSEA) Nominee training program held at National Institute of Animal Walfare, Ballabhgarh Faridabad from 14th – 18th January,2013.

 

 

List of Publications

 
1. Khanna M, Saxena L, Gupta S, Gaur SN,(2015) A Focus on Respiratory Tract Infection as an Important Risk Factor in COPD. Vol. 1 No. 2:8

 

 

2. Saxena L, Khanna M, (2015). Production and Characterization of Human Monoclonal Antibodies from the Cells of A(H1N1)pdm2009 Influenza Virus Infected Indian Donors. Procedia in Vaccinology 9 50 – 58

 

 

3. Rajput R, Sharma G, Rawat V, Gautam A, Kumar B, Pattnaik B, Pradhan HK, Khanna M. (2015). Diagnostic Potential of Recombinant scFv Antibodies Generated against Hemagglutinin Protein of Influenza a Virus. Front Immunol. Sep 2;6:440. doi: 10.3389/fimmu.2015.00440.

 

 

4. Kumar B, Rajput R, Pati DR, Khanna M (2015). Potent Intracellular Knock-Down of Influenza A Virus M2 Gene Transcript by DNAzymes Considerably Reduces Viral Replication in Host Cells. Mol Biotechnol. 2015 Sep;57 (9):836-45. doi: 10.1007/s12033-015-9876-z.

 

 

5. Khanna M, Saxena L, Rajput R, Kumar B, Prasad R. (2015). Gene silencing: a therapeutic approach to combat influenza virus infections. Future Microbiol. 10(1):131-40. doi: 10.2217/fmb.14.94.

 

 

6.Khanna M, Rajput R, Kumar B, Pati DR, Prasad R. (2014). Respiratory Viral Infections in Exacerbations of Chronic Obstructive Pulmonary Disease. BioScience Trends. Under review.

 

 

7. Khanna M, Rajput R, Kumar B, Singh A, Saxena L. (2014). Influenza virus induced cytokine responses: An evaluation of host-pathogen association. Immunome Research. 10: S2: 001. doi.org/10.4172/1745-7580.S2.001.

 

 

8. Khanna M, Sharma S, Kumar B, Rajput R. (2014). Protective Immunity Based on the Conserved Hemagglutinin Stalk Domain and Its Prospects for Universal Influenza Vaccine Development. BioMed Research International.  DOI: 10.1155/2014/546274

 

 

9. Rajput R, Khanna M & Pradhan HK. (2014). Phage-display technology for production of recombinant monoclonal antibodies. Materials and Methods. 4:873. http://dx.doi.org/10.13070/mm.en.4.873.

 

    10. Kumar B, Kumar P, Rajput R, Saxena L, Daga MK, Khanna M. (2013).  Sequence-specific cleavage of BM2 gene transcript of influenza B virus by 10-23 catalytic motif containing DNA enzymes significantly inhibits viral RNA translation and replication. Nucleic Acid Ther. doi: 10.1089/nat.2013.0432.

 

11. Kumar P, Kumar B, Rajput R, Saxena L, Banerjea AC and Khanna M. (2013). Cross-protective effect of antisense oligonucleotide developed against the common 3’ NCR of influenza A virus genome. Mol. Biotechnol. DOI 10.1007/s12033-013-9670-8

 

12. Pati DR, Khanna M, Kumar B, Kumar P, Rajput R, Saxena L, Sharvani, Gaur SN. (2013). Clinical presentation of patients with seasonal influenza and pandemic influenza a (H1N1-2009) requiring hospitalization. Indian J Chest Dis Allied Sci; 55:15-19.

 

13. Khanna M, Saxena L, Gupta A, Kumar B & Rajput R. (2013). Influenza pandemics of 1918 and 2009: a comparative account. Future Virology. 8(4):335-342

 

14. Rajput R, Khanna M, Kumar P, Kumar B, Sharma S, Gupta N, Saxena L. (2012). siRNA targeting the nonstructural gene (NS1) transcript inhibits influenza A virus replication in experimental mice. Nucleic Acid Ther. 22(6): 414-22.

 

15. Srivastava V, Khanna M, Sharma S and Kumar B (2012). Resolution of Immune Response by recombinant transforming growth factor-beta (rTGF-β) during influenza A virus infection. IJMR 136: 641-648.

 

16. Kumar P, Khanna M, Kumar B, Rajput R, Banerjea A.C (2012).A conserved matrix epitope based DNA vaccine protects mice against influenza A virus challenge. Antiviral Res. 93:78-85.

 

17. Kumar B, Khanna M, Kumar P, Sood V, Vyas R, Banerjea A.C. (2012). Nucleic Acid Mediated Cleavage of M1 Gene of Influenza A Virus Is Significantly Augmented by Antisense Molecules Targeted to Hybridize Close to the Cleavage Site. Mol. Biotechnol. 51:27-36.

 

18. Kumar P, Vyas R, Sood V, Gupta N, Banerjea A & Khanna M (2010). Potent    inhibition of influenza virus replication with novel siRNA-chimeric-ribozyme constructs. Antiviral Res. 87: 104-112.

 

19. Khanna M, Rajput R, Kumar P, Sharma D, Mathur D, Prasad AK (2011). Potent inhibition of pandemic influenza H1N1 (2009) virus propagation by novel chemically synthesized compounds. Inf & Resp Viruses 5 (Suppl.1), 90-123.

 

20. Kumar B, Pati D.R, Khanna M, Kumar, P, Daga M.K, Singh V, Khare S, Gaur S.N (2012) Age-sex distribution and seasonality pattern among influenza virus infected patients in Delhi, 2009-2010. Ind. J Com. Med. 37:57-58.

 

21. Khanna M, Kumar B, Gupta  A, Kumar  P. (2012) Pandemic influenza A H1N1 (2009) virus: Lessons from the past and implications for the future,  Ind J Virol.Volume 23Issue 1, 12-17.

 

22. Kumar B, Khanna  M, Kumar P, Gupta  A, Daga M.K, Sarkar M.C, Chadha M.S, Mishra A.C, Kaur H (2011). Quantification of viral load in clinical specimens collected from different body sites of patients infected with influenza viruses. Int. J. Med. Med. Sci. 3 (5):144-148.

 

23. Kumar B, Sharma B, Khanna M, Singh V, Daga MK, Vijayan VK, Mishra AC, Chadha MS, Sarkar MC & Kaur H (2010). Comparison of various immunoassay kits for rapid screening of pandemic influenza H1N1-2009 viruses. J. Pub. H Epidemiol 2:175-179.

 

24. Kumar P., Kumar B., Gupta A., Sharma B., Vijayan V.K., Khare S., Singh V., Daga M.K., Chadha M.S., Mishra A.C., Kaur H., Khanna M (2010). Diagnosis of novel pandemic influenza virus 2009 H1N1 in hospitalized patients. Ind J Viro. 21:45-49.

 

25. Khanna M, Ray A, Rawall S, Chandna S, Kumar B and Vijayan VK (2010). Detection of Influenza virus induced ultrasructural changes and DNA damage; Ind J Virol. 21:50-55.

 

26. Khanna M and Gupta A (2010). Vaccines for Pandemic Influenza A H1N1 (2009): Where Do We Stand? Ind J Virol.21:90-91.

 

27. Khanna M, Kumar B, Gupta N, Kumar P, Vijayan VK and Kaur H (2009). Pandemic swine influenza virus (H1N1): a threatening evolution; Ind J Microbiol 49:365–369.

 

28. V. Srivastava, S. Rawall, V.K. Vijayan & M. Khanna (2009) Influenza A virus induced apoptosis: Inhibition of DNA laddering & caspase 3 activity by zinc supplementation in cultured HeLa cells IJMR 129, pp 115-122.

 

29. Khanna M, Kumar P K Chaudhry,B Kumar & V.K. Vijayan (2008) Emerging influenza virus: A Global Threat J Bio Sci; 33:475-482 .

 

30. Khanna M,Gupta N,Gupta A & vijayan VK (2009).  Influenza 2009 H1N1 :a pandemic alarm J Bio Sci 34(3), September 2009, 481–489.

 

31. Nazir R, Khanna M & Kulshrestha R. Time Course of Pulmonary Pathology, Cytokine Influx and Their Correlation on Augmentation of Antigen Challenge by Influenza A Virus Infection. IJEB. 2008; 46: 151-158.

 

32. Kumar P,  Srivastava V, Khanna M  and Vijayan V K, (2007)  Biphasic effect of immune r TGF-β1 on  inducible Nitric oxide Synthase during Influenza A virus infection in mice.  Proceedings of Option for Control of Influenza 559-560.

 

33. Khanna M, Kumar P, Vijayan V K, Singh V. (2007) Release of Macrophage inflammatory protein, IL-6 and Eosinophilic derived neurotoxin into upper respiratory secretions during influenza virus infection in children Ind J Chest Dis 49: 249-251.

 

34. Kumar P, Khanna M, Tyagi Y.K, Srivastava V, Ravi K & Raj H.G. (2005) Effect of Quercetin supplementation on   antioxidant of lung after experimental influenza virus infection. Exp. Lung Res. 31 (5).

 

35. Kumar P, Khanna M, Sharma S and Raj HG. (2003). Effect of Quercetin on    lipid peroxidation and changes in lung morphology in experimental influenza virus infection. Int. J. Exp. Path. 84; 1-7.

 

36. Khanna M, Kumar P, Chhabra SK, Chugh P and Prasad AK (2001).  Evaluation of influenza virus detection by direct enzyme immunoassay (EIA) and conventional methods in asthmatic patients. J Commun Dis 33; 163-9.

 

37. Khanna M, Akhter N, Srivastava V, Kumar P Vijayan V.K., (2006) Biological and Epidemiological Aspects of Influenza Virus H5N1 in Context of India. Ind. J. Exp. Biol., 44: 265-278.

 

38. Khanna M, Kumar P. (2002). Influenza: A serious global threat. J. Infect. Dis. Antimicro. Agents. 19; 25-39.

 

39. Khanna M, Srivastava V and Kumar P. (2002). Virological studies in clinical practice: Utility & Indications. Medicine Update Annual Ed. 7/2; 74-78.

 

40. Khanna M, Kumar P and Prasad AK. Influenza and its role in apoptosis. (2001). IJAAI. 15; 7-12.

 

41. Khanna M, Kumar P, Srivastava LM. (2003) Defective Interlukin-2 and IL-2 receptor expression in pulmonary tuberculosis. J Commun Dis. 35 (2) 65-70.

 

42. Sharma S.K., Khilnani,V. Pandey.V.C. and Khanna, M.(1995). Immunological and Pulmonary Function Abnormalties in tropical Pulmonary Eosinophilia. IJMR, 101:98-102.

 

43. Sharma SK, Khanna M, Srivastava LM, Pandey JC and Khilnani V. (1995). Effect of corticosteriod treatment on various serological and bronchoalveolar lavage (BAL) abnormalties in patients with sarcoidosis. IJMR. 101:207-212.

 

44. Khanna M and Srivastava LM. (1996) Release of superoxide anion from activated mouse peritoneal macrophages during Mycobacterium tuberculosis infection. Ind. J. Exp. Biol.  34, 407-410.

 

45. Khanna M, Goel A, Jain RC, and Srivastava LM. (1997). Rapid and simple method for detection of rifampicin resistant clinical isolates of M.  Tuberculosis. Ind. J. Clin. Biochem. 12 (suppl); 83-85.

 

46. Khanna M, Chaturvedi UC and Mathur A. (1988).  Abrogation of helper T cell by dengue virus induced cytotoxic factor. Current Science. 57; 411-414.

 

47. Khanna M, Chaturvedi UC and Mathur A. (1989). Cytotoxic effects   of dengue virus induced cytotoxin on helper T cell.  Ind. J. Med. Micro. 7; 54-57.

 

48. Khanna M, Chaturvedi UC and Mathur A. (1989b). Proteinase like activity in the cytotoxic factor produced by T cells during dengue virus infection. Immunology. 62; 32-37.

 

49. Dhawan R, Khanna M, Chaturvedi UC and Mathur A. (1990). Effects of dengue virus-induced cytoxin on capillary permeability. Br. J.Exp.Path. 71; 83-89.

 

50. Khanna M, Chaturvedi UC and Mathur A. (1990). Increased capillary permeability mediated by dengue virus-induced lymphokine. Immunology. 69; 449-453.

 

51. Dhawan R, Khanna M, Chaturvedi UC and Mathur A. (1990) Role of calcium in induction and secretion dengue virus-induced cytokines. J. Bio. Sci. 15; 409-416.

 

52. Dhawan R, Chaturvedi UC, Khanna M, Mathur A, Tekwani BL, Pandey VC and Rai RN. (1991). Obligatory role of calcium in the cytotoxic activity of dengue virus-induced cytotoxin.  Int.  J.  Exp. Pathol. 72; 31-39.

 

53. Khanna M, Chaturvedi UC, Dhawan R, Tekwani BL and Pandey VC. (1991). Presence of calcium is obligatory for the cytotoxic activity of dengue virus-induced cytotoxic factor.  Immunology. 72; 73-78. 

 

54. Chaturvedi UC, Dhawan R and Khanna M. (1991). Breakdown of blood brain barrier by dengue virus-induced cytotoxic factor. J Gen. Virology. 72; 859-866.

 

55. Khanna M and Chaturvedi UC. (1992). Purification and amino acid terminal sequence of the dengue virus-induced cytotoxic factor. Br. J Expt. Pathol. 73; 43-49.

 

56. Dhawan R, Chaturvedi UC and Khanna M.  (1994). Breakage of blood brain barrier    by macrophage cytotoxin. Proc. Indian Natl. Sci. Acad. 60; 45-52.

 

 

International book Chapters

 

1. Khanna M, Saxena L, Rajput R, Sharma S, Kumar B. Biotechnology in development of viral markers.532-550.In: Biotechnology in Medicine and Herbal Drug Development, Gill SS, Singh Z, Bansal P, eds.  Basera Verlag, Germany. (2014) ISBN: 978-81-92064-0-4.

 

2. Khanna M, Saxena L. RNA interference and its applications in human health.161-170.In: Recent Advances in Assisted Reproductive Technology. Gill SS, Singh Z, Bansal P, eds. Gulab Publishers, Sangur, India. (2013) ISBN: 978-81-92064-0-6.

 

3. Khanna M, Srivastava V., Structure and Classification of Influenza Virus. P 11 – 23. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6).

 

4.  Khanna M, Srivastava V., Clinical and Laboratory Diagnosis of Influenza Virus. P 138 – 151. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6).

 

5.  Khanna M, Nazir R, Srivastava V., Prophylaxis Prevention and Treatment for Influenza A,B,C. p 169 – 191. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6).

 

6.  Khanna M, Srivastava V., Influenza Pandemic Preparedness. P 192 – 201. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6).

 

 

Chapters in national books

 

1.  Khanna M, Saxena L. RNA interference and its applications in human health.161-170.In: Recent Advances in Assisted Reproductive Technology. Gill SS, Singh Z, Bansal P, eds. Gulab Publishers, Sangur, India. (2013) ISBN: 978-81-92064-0-6.